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Anglers are reporting fish ranging from 2lb to 5lb, especially on dry flies, fished during the hatch window which is normally mid-afternoon
This simple mechanism is what experts call selectivity
Purified EVs were lysed on ice in radio-immunoprecipitation assay (RIPA) buffer (Thermo Fisher Scientific) containing 1 protease inhibitor and 1 mM phenylmethylsulfonyl fluoride (PMSF)
Another factor linked to water clarity is leader diameter, I generally use Fulling Mill fluorocarbon in 5lb, 6lb and 7lb
