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Some wrecks inside the site may have been buried by the rock deposits

EV quantification by flow cytometry Following SEC fraction collection, EVs were labeled with anti-human BV785-conjugated CD11b antibody (Clone: M1/70, 1:1000 dilution, BioLegend), FITC-conjugated annexin V (1:1000 dilution, BioLegend), and anti-human PE-conjugated CD9 (Clone: MM2/57, 1:500 dilution, SouthernBiotech) for 20 min at 21 C and then washed in HBSS +/+ (repeated centrifugation with 16,100 g, 20 min, 4 C)

So 15-pound test fluorocarbon makes for a pretty good line selection here

Leader too long for the wind A 12 leader can be great on calm days, but it can collapse in wind and ruin turnover